FastQCFastQC Report
Tue 25 Jul 2017
AdapterDimer_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameAdapterDimer_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37969586
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC681480117.948051896062285TruSeq Adapter, Index 11 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC4414781.162714810743525TruSeq Adapter, Index 11 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAGCTCGTATGC969490.2553333080850552TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACACCTCGTATGC954260.25132220298635866TruSeq Adapter, Index 11 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACAACTCGTATGC643730.1695383247001956TruSeq Adapter, Index 11 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA17605550.062.985529
AGAGCAC18234800.060.953748
AAGAGCA19038600.058.4854287
GAAGAGC19541300.056.9142656
GGAAGAG20224650.054.9816325
CGGAAGA20787350.053.5615464
TCGGAAG21181550.052.626533
ATCGGAA21657550.051.550992
GATCGGA21716750.051.2532921
GCTTGAA8467850.024.298655-59
CTGCTTG9348000.023.89999455-59
TGAAAAA8599800.023.78693260-64
TCTTCTG9419750.023.72929650-54
TGCTTGA8718100.023.70104855-59
TCTGCTT9401700.023.65259255-59
GCCGTCT9513250.023.6325145-49
TGCCGTC9574450.023.6220745-49
ATGCCGT9939900.023.58221845-49
CTTCTGC9537200.023.53611450-54
GTCTTCT9528150.023.38618750-54
Produced by FastQC (version 0.11.5)