FastQCFastQC Report
Wed 11 Feb 2015
MethylSeq_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMethylSeq_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19376807
Sequences flagged as poor quality0
Sequence length150
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGGT13100.0103.919081
CTGTGGT32700.0101.3245851
CAGTGGT16350.0100.4435041
CTTGGGT37600.098.656151
CTATGGT18400.098.647821
CCATGGT5500.098.220781
CCGTAGT9950.097.72721
CTCTGGT8350.097.4757841
CCTAGGT10400.096.268951
CCTTGGT8050.095.739841
CCGTGGT14500.094.382261
CTTAGGT26700.092.261311
CCGAGGT9150.092.10211
CAATGGT8900.090.642691
ACATGGT7650.089.447241
CATGGGT17100.089.298581
CATAGGT14000.089.006731
CTTGTGT16550.084.867491
CTGATGT15700.084.415651
CTATGGG15400.083.25381
Produced by FastQC (version 0.11.2)