FastQCFastQC Report
Sat 25 Mar 2017
SmallRNASeq_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSmallRNASeq_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8209629
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per base N content

N content graph

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA4967146.050383031925072RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG4581565.580715035965693RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2871753.4980265247065363RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC2254022.7455808295356587No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT2054962.5031094584176703RNA PCR Primer, Index 1 (100% over 30bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2001232.437661921141625RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1725972.10237271379742RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA1647402.0066680236098366RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCGTGGAATTCTCGGGTGCCAAGGAACTCCA1496251.822554953457702RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1322041.6103529160696546No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1263881.5395092762413503No Hit
GTTTCCGTAGTGTAGCGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1111121.3534350943264306No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA998691.2164861530283524RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC848461.0334937181692376RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC680860.8293432017451702RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC676690.8242638004713733RNA PCR Primer, Index 1 (100% over 26bp)
CGCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT673600.8204999275850345RNA PCR Primer, Index 4 (100% over 43bp)
TGAGAACTGAATTCCATGGACTGTGGAATTCTCGGGTGCCAAGGAACTCC666150.8114252178752536RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC584600.7120906438037578RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC575130.7005554087766939RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA525100.6396147743095333RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTGGAATTCTCGGGTGC513080.6249734305898598No Hit
TGAAATGTTTAGGACCACTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG469430.5718041582634246RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA466180.5678453922826476RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCGTTGGAATTCTCGGGTGCCAAGGAACTCC460410.5608170600644682RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC458740.5587828633912689No Hit
GTGAAATGTTTAGGACCACTTGTGGAATTCTCGGGTGCCAAGGAACTCCA433870.5284891680245234RNA PCR Primer, Index 1 (100% over 28bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA430290.5241284350364676RNA PCR Primer, Index 1 (100% over 28bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG423120.5153947882419534RNA PCR Primer, Index 4 (100% over 45bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA399720.48689167318035936No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC383540.46718310900529125RNA PCR Primer, Index 1 (100% over 27bp)
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA351420.4280583202968124RNA PCR Primer, Index 1 (100% over 28bp)
TCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT350780.42727874791905945RNA PCR Primer, Index 4 (100% over 43bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA343790.41876435585578836RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA341740.4162672880832983No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA340780.4150979295166688RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT328740.4004322241601905RNA PCR Primer, Index 1 (100% over 30bp)
TGAGAACTGAATTCCATGGACTGTTGGAATTCTCGGGTGCCAAGGAACTC328080.39962829014563267RNA PCR Primer, Index 1 (100% over 26bp)
ACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTCC321670.3918203855497003RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCA321290.39135751445040945RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG285580.34785981193547233RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAAG282060.34357216385783085No Hit
TGAGATGAAGCACTGTAGCTCTTTGGAATTCTCGGGTGCCAAGGAACTCC276530.3368361712813088RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGATCCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTC275240.33526484570740045RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC264710.32243844392968307No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG264060.32164669073352764RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA251470.30631104036491785No Hit
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC246090.2997577600644316RNA PCR Primer, Index 4 (100% over 44bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC240340.292753789483057No Hit
CCCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT232430.283118762126766RNA PCR Primer, Index 4 (100% over 43bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA231320.28176669128410065No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG229120.2790869112355747RNA PCR Primer, Index 1 (100% over 29bp)
CCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCT215670.26270371048435925RNA PCR Primer, Index 4 (100% over 43bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC215490.26248445575311624RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA203850.24830598313273353RNA PCR Primer, Index 1 (100% over 28bp)
ACAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG192810.23485835961649426RNA PCR Primer, Index 1 (100% over 29bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCC190970.23261708903045433RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGACTCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA188660.22980331997950212RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCTGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA185620.22610035118517535No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA184380.2245899297032789RNA PCR Primer, Index 1 (100% over 28bp)
TCAGACAACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA179780.2189867532381792RNA PCR Primer, Index 1 (100% over 32bp)
TGAGAACTGAATTCCATGGACTTGGAATTCTCGGGTGCCAAGGAACTCCA176230.21466256270533052RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTGATGGAATTCTCGGGTGCCAAGGAACTC151340.18434450570178018RNA PCR Primer, Index 1 (100% over 26bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC149080.1815916407428399RNA PCR Primer, Index 4 (100% over 44bp)
ACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG148420.1807877067282821RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC144560.17608591082495934RNA PCR Primer, Index 1 (100% over 26bp)
GTTTCTCTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC142000.17296762131394733No Hit
TACCCTGTAGAACCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACTC141820.1727483665827043RNA PCR Primer, Index 1 (100% over 26bp)
TACAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA140030.17056800008867634RNA PCR Primer, Index 1 (100% over 28bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC131050.15962962516332954RNA PCR Primer, Index 4 (100% over 44bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC127890.1557804865481741RNA PCR Primer, Index 1 (100% over 27bp)
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCC125140.15243076148751666RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA124870.15210187939065214No Hit
TACCCTGTAGATCCGAATTTGTGTTGGAATTCTCGGGTGCCAAGGAACTC121540.14804566686265605RNA PCR Primer, Index 1 (100% over 26bp)
TGAAATGTTTAGGACCACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA120940.1473148177585126RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC119830.14596274691584724RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCTGGAATTCTCGGGTGCCAAGGAA117870.14357530650897865RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCAA117850.14355094487217387No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG117130.14267392594720177No Hit
GTTTCTGTAGTGTAGTGGTTATCACGTTCGCCATGGAATTCTCGGGTGCC114860.13990888016985906No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC112850.13746053567097855No Hit
ACCACAGGGTAGAACCACGGACTGGAATTCTCGGGTGCCAAGGAACTCCA111300.135572508818608RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC110580.1346954898936359No Hit
GCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC105350.12832492186918557RNA PCR Primer, Index 4 (100% over 44bp)
CTGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCA104750.12759407276504212RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCTGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC104680.12750880703622539No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC103580.12616891701196242RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGAATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA99110.1207240911860938RNA PCR Primer, Index 1 (100% over 28bp)
CTGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCC97140.11832446996082283RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA97140.11832446996082283RNA PCR Primer, Index 1 (100% over 28bp)
GTACAGTACTGTGATAACTGATGGAATTCTCGGGTGCCAAGGAACTCCAG96220.1172038346678029RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG95670.1165338896556714RNA PCR Primer, Index 1 (100% over 29bp)
CCGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC95400.11620500755880686RNA PCR Primer, Index 4 (100% over 44bp)
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCG93110.1134156001446594RNA PCR Primer, Index 4 (100% over 45bp)
GTTTCCGTAGTGTAGCGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA91850.11188081702595819No Hit
TCAGTGCACTACAGAACTTTGTTTGGAATTCTCGGGTGCCAAGGAACTCC91430.11136922265305776RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC91060.11091853237216931No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC89210.10866508096772705RNA PCR Primer, Index 4 (100% over 50bp)
GTCCAGTTTTCCCAGGAATCCCTTGGAATTCTCGGGTGCCAAGGAACTCC89040.1084580070548864RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACTTTCGCCTTGGAATTCTCGGGTGCC86740.10565641882233656No Hit
TGTAAACATCCTCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC86690.1055955147303246RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT85620.10429216716126881RNA PCR Primer, Index 1 (100% over 30bp)
GACAGTCAGCAGTTGGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG85610.10427998634286642RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTTGGAATTCTCGGGTGCCAAGGAACTCCA83710.10196563084641218RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG82780.10083281473498985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTTA351.4036596E-744.172651
TTAGTGC254.343655E-544.172651
TATTGCA105850.044.151781
TCCTTCA79200.044.0332151
CCAGGTT302.5196077E-644.025383
TTACGTA302.5196077E-644.025383
AACATTC158400.044.019271
TACCCTG729700.043.9970971
ATAATCC800.043.996156
TTATCTA207.862697E-443.996158
AAGTTTA207.862697E-443.996155
AATCTAC351.448534E-743.996157
CTCATAG700.043.996158
ATCATCC254.4475346E-543.996155
GGCCTAC302.5311492E-643.996155
TACAACG408.3291525E-943.996155
ACGCGTC502.7284841E-1143.996158
GGCTTAC454.802132E-1043.9961475
CCATAGT2850.043.9961475
CCCTTAT1250.043.99561332
Produced by FastQC (version 0.11.5)